Principle of SNAP/CLIP-labeling and labeling protocols. (a) SNAP-and

Download scientific diagram | Principle of SNAP/CLIP-labeling and labeling protocols. (a) SNAP-and CLIP-tag fused to the protein of interest bind benzylguanine-or benzylcytosine-substrates, respectively. There are various cell-permeable fluorescent substrates and a non-fluorescent substrate available. (b) For labeling of the whole fusion protein pool cells are incubated with a fluorescent substrate, washed and fixed/imaged. (c) For age-dependent labelings cells are first incubated with the non-fluorescent blocker followed by labeling of the newly-synthesized fusion protein with a fluorescent substrate. To omit continuous labeling with the first substrate additional blocking/ labeling steps can be added.   from publication: A Global Approach for Quantitative Super Resolution and Electron Microscopy on Cryo and Epoxy Sections Using Self-labeling Protein Tags | Correlative light and electron microscopy (CLEM) is a powerful approach to investigate the molecular ultrastructure of labeled cell compartments. However, quantitative CLEM studies are rare, mainly due to small sample sizes and the sensitivity of fluorescent proteins to | Protein Tagging, Electron Microscopy and Correlative Light Electron Microscopy | ResearchGate, the professional network for scientists.

Fluorescent Labeling Of COS-7 Expressing SNAP-tag Fusion Proteins l Protocol Preview

SNAP/CLIP-Tags and Strain-Promoted Azide–Alkyne Cycloaddition (SPAAC)/Inverse Electron Demand Diels–Alder (IEDDA) for Intracellular Orthogonal/Bioorthogonal Labeling

PDF) A Global Approach for Quantitative Super Resolution and Electron Microscopy on Cryo and Epoxy Sections Using Self-labeling Protein Tags

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PDF) A Global Approach for Quantitative Super Resolution and Electron Microscopy on Cryo and Epoxy Sections Using Self-labeling Protein Tags

Principle of SNAP/CLIP-labeling and labeling protocols. (a) SNAP-and

Jean-Marc VERBAVATZ, Group Leader, Institut Jacques Monod, Paris, IJM

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Principle of SNAP/CLIP-labeling and labeling protocols. (a) SNAP-and

Beyond O6-Benzylguanine: O6-(5-Pyridylmethyl)guanine as a Substrate for the Self-Labeling Enzyme SNAP-Tag

Jean-Marc VERBAVATZ, Group Leader, Institut Jacques Monod, Paris, IJM

SNAP/CLIP-Tags and Strain-Promoted Azide–Alkyne Cycloaddition (SPAAC)/Inverse Electron Demand Diels–Alder (IEDDA) for Intracellular Orthogonal/Bioorthogonal Labeling

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