Chemical heater-based, instrumentation-free molecular detection of

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Download scientific diagram | Chemical heater-based, instrumentation-free molecular detection of SARS-Cov-2 in saliva. (A) Test workflow: (1) collect saliva sample in a tube; (2) mix saliva with lysis buffer; (3) aliquot the sample to individual tubes, each dry-storing an RT-LAMP reaction mix specific to the selected target; (4) insert tube(s) in the chemical heater and add water to the EPCM to initiate an exothermic reaction and heat the tubes to 60-65 °C for ∼30 minutes. (B) Color change of the tube indicates whether the test is positive (yellow, three tubes) or negative (red, one tube). Here, the negative test is a negative control to verify that the color change does not occur because of the chemical composition of the sample. from publication: Electricity-free chemical heater for isothermal nucleic acid amplification with applications in COVID-19 home testing | Molecular detection of pathogenic nucleic acids from patient samples requires incubating biochemical reactions at specific temperatures to amplify DNA. This incubation is typically carried out with an electrical heater and a temperature controller. To reduce test cost, to | COVID-19, Nucleic Acids and Nucleic Acid Amplification Techniques | ResearchGate, the professional network for scientists.